Surface pH controls purple-to-blue transition of bacteriorhodopsin. A theoretical model of purple membrane surface
نویسندگان
چکیده
منابع مشابه
Effect of lipid surface charges on the purple-to-blue transition of bacteriorhodopsin.
Purple membrane (lambda max = 568 nm) can be converted to blue membrane (lambda max = 605 nm) by either acid titration or deionization. Partially delipidated purple membrane, containing only 25% of the initial lipid phosphorus, could be converted to a blue form by acid titration but not by deionization. This reversible transition of delipidated membrane did not require the presence of other cat...
متن کاملProtonation dynamics of the extracellular and cytoplasmic surface of bacteriorhodopsin in the purple membrane.
The dynamics of proton binding to the extracellular and the cytoplasmic surfaces of the purple membrane were measured by laser-induced proton pulses. Purple membranes, selectively labeled by fluorescein at Lys-129 of bacteriorhodopsin, were pulsed by protons released in the aqueous bulk from excited pyranine (8-hydroxy-1,3,6-pyrenetrisulfonate) and the reaction of protons with the indicators wa...
متن کاملChromophore of Bacteriorhodopsin is Closer to the Cytoplasmic Surface of Purple Membrane: Fluorescence Energy Transfer on Oriented Membrane Sheets.
Transmembrane location of the retinal chromophore, either native or reduced in situ to a fluorescent derivative, of the purple membrane of Halobacterium halobium was investigated with fluorescence energy transfer techniques. Single sheets of purple membrane, either native or reduced with borohydride, were adsorbed on polylysine-coated glass; the orientation, whether the exposed surfaces were cy...
متن کاملThe effect of lipid environment in purple membrane on bacteriorhodopsin.
The decay rate of the Bacteriorhodopsin (BR) photocycle intermediate M412 and proton, the proton pump efficiency (H+/M412), the ratios of M412 to other intermediates and the rotational correlation time (tauc) in purple membrane (PM) fragments treated by the zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) with different concentrations were studied. The r...
متن کاملStructural changes of purple membrane and bacteriorhodopsin during its denaturation induced by high pH.
Bacteriorhodopsin (bR) trimers naturally form two-dimensional hexagonal crystals in purple membrane (PM), which make it very stable. However, the dnaturation of bR was found to occur during a very narrow pH range when the pH was increased above 12.0, as indicated by inactivation of the photochemical cycle observed by flash photolysis kinetic spectra. Here, atomic force microscopy was used to st...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1989
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(89)82683-9